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nb100 56509 wb  (Novus Biologicals)


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    Novus Biologicals nb100 56509 wb
    Nb100 56509 Wb, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nb100+56509+wb/IKK+beta+Antibody+(10AG2)+-+BSA+Free/10__7554_slash_elife__98009__4-263-163-160
    Average 92 stars, based on 17 article reviews
    nb100 56509 wb - by Bioz Stars, 2026-09
    92/100 stars

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    Article Title: Dual-specific autophosphorylation of kinase IKK2 enables phosphorylation of substrate IκBα through a phosphoenzyme intermediate
    Article Snippet: .. Reagent type (species) or resource Designation Source or reference Identifiers Additional information Gene (Homo sapiens) IKBKB GenBank Gene ID: 3551 Gene (Homo sapiens) NFKBIA GenBank Gene ID: 4792 Gene (Homo sapiens) IKBKG GenBank Gene ID: 8517 Strain, strain background (Escherichia coli) Rosetta2 (DE3) Other Chemically competent cells Strain, strain background (Escherichia coli) DH5α New England Biolabs Cat# C2987H Chemically competent cells Strain, strain background (Spodoptera frugiperda) Sf9 cells Thermo Fischer Cat# 12659017 Antibody Anti- IκBα (L35A5) (Mouse monoclonal) Cell Signaling Technology Cat# 4814, RRID:AB_390781 WB (1:2000) Antibody Anti- PhosphoIκBα (Ser32/36) (5A5) (Mouse monoclonal) Cell Signaling Technology Cat# 9246, RRID:AB_2267145 WB (1:2500) Antibody Anti- PhosphoIKKα/β (Ser176/180) (16A6) (Rabbit monoclonal) Cell Signaling Technology Cat# 2697, RRID:AB_2079382 WB (1:2000) Antibody Anti- PhosphoTyrosine (Mouse monoclonal) BD Biosciences Cat# 610000, RRID:AB_397423 WB (1:500) Antibody Anti- PhosphoTyrosine (Mouse monoclonal) EMD Millipore Cat# 05–321 X WB (1:1000) Antibody Anti- IKKβ (Rabbit Polyclonal) Bio Bharti Life Science Cat# BB- AB0094 WB (1:2000) Antibody Anti- IKKβ (10AG2) (Mouse monoclonal) Novus Biologicals Cat# NB100- 56509 WB (1:5000) Borar et al. eLife 2024;13:RP98009. .. DOI: https://doi.org/10.7554/eLife.98009 17 of 29 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti- 6XHis (Rabbit Polyclonal) Bio Bharti Life Science Cat# BB- AB0010 WB (1:2500) Antibody Anti- GST (Rabbit polyclonal) BioLegend Cat# 924801, RRID:AB_2565461 WB (1:10000) Antibody Anti- PhosphoSerine Q5 (Mouse Monoclonal) Qiagen Cat# 37430 WB (1:2000) Antibody Anti- NEMO (Mouse monoclonal) BD biosciences Cat# 559675, RRID:AB_397297 IP (1:1AB_397297AB_397297AB_397297AB_397297AB_39729700) Antibody Anti- HA 11 epitope (Mouse monoclonal) BioLegend Cat# 901502, RRID:AB_2565007 WB (1:2000) IP (1:100) Antibody Anti- Tubulinβ3 (Mouse monoclonal) BioLegend Cat# 657402, RRID:AB_2562570 WB (1:10,000) Recombinant DNA reagent pFastBac HT B (plasmid) Invitrogen Recombinant DNA reagent pET24d (6xHisTEV) (plasmid) (modified) Other Prepared in lab Sequence- based reagent IKK2_1_F This paper PCR primer 5’- GCAT GATC AAGC TG GTCA CCTT CCCT GAC-3’ Sequence- based reagent IKK2_700_R This paper PCR primer 5’- ATAA GAAT GCGG CCG CTCA CTCA GGTA AGCT GTTG GAGG CCG-3’ Sequence- based reagent IKK2_AA_F This paper PCR primer 5’- GCCA AGGA GCTG GAT CAGG GCGC TCTT TGCA CAGC ATTC GTGG GGAC CCTG CAGT AC-3’ Sequence- based reagent IKK2_AA_R This paper PCR primer 5’- GTAC TGCA GGGT CCC CACG AATG CTGT GCAA AGAG CGCC CTGA TCCA GCTC CTTG GC-3’ Sequence- based reagent IKK2_Y169F_F This paper PCR primer 5’- CACA AAAT TATT GACC TAGG ATTT GCCA AGGA GCTG GATC AGGG C-3’ Sequence- based reagent IKK2_Y169F_R This paper PCR primer 5’- GCCC TGAT CCAG CTC CTTG GCAA ATCC TAGG TCAA TAAT TTTG TG-3’ Sequence- based reagent NEMO_F This paper PCR primer 5’- CGGA ATTC ATG AATA GGCA CCTC TGG-3’ Sequence- based reagent NEMO_R This paper PCR primer 5’- ACGC GTCG ACTC A CTCA ATGC ACTC CATG -3’ Sequence- based reagent IκBα_F This paper PCR primer 5’- CGCG GATC CATG TT CCAG GCGG CCGA G-3’ Sequence- based reagent IκBα_R This paper PCR primer 5’- ACGC GTCG ACTC AT AACG TCAG ACGC TG-3’ Peptide, recombinant protein FL IKK2 WT (Polley et al., 2013) PMID:23776406 Sf9- baculovirus expression system Peptide, recombinant protein FL IKK2 K44M This paper Sf9- baculovirus expression system Continued on next page Continued Borar et al. eLife 2024;13:RP98009.



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    nb100 56509 wb  (Novus Biologicals)
    92
    Novus Biologicals nb100 56509 wb
    Nb100 56509 Wb, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nb100+56509+wb/IKK+beta+Antibody+(10AG2)+-+BSA+Free/10__7554_slash_elife__98009__4-263-163-160
    Average 92 stars, based on 1 article reviews
    nb100 56509 wb - by Bioz Stars, 2026-09
    92/100 stars
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